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International Journal of Zoology and Applied Biosciences Research Article
White analytical chemistry driven green and sensitive UV spectroscopic method for estimation of Risankizumab
Harini Upppada, Moni Prasanna Mandalapu, Tanusree Muvvala, Yamini Munakala, Kavya Maddela, Naveena Koyya and Jagadeesh Panda
Year : 2025 | Pages: 705-714
Received on: 11/10/2025
Revised on: 28/10/2025
Accepted on: 27/11/2025
Published on: 01/12/2025
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Harini Upppada, Moni Prasanna Mandalapu, Tanusree Muvvala, Yamini Munakala, Kavya Maddela, Naveena Koyya and Jagadeesh Panda( 2025).
White analytical chemistry driven green and sensitive UV spectroscopic method for estimation of Risankizumab
. International Journal of Zoology and Applied Biosciences, 10( 6), 705-714.
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Abstract
Risankizumab is a humanized monoclonal antibody used for the treatment of autoimmune diseases such as plaque psoriasis. A simple, sensitive and precise UV spectroscopic method was developed and validated for the quantification of Risankizumab in pharmaceutical formulations. The maximum absorbance, was obtained at 289 nm. The validation parameters, including linearity, accuracy, precision, and robustness, were evaluated according to the International Conference on Harmonisation (ICH) guidelines. The method showed excellent linearity (R² > 0.999) over the concentration range of 20-100 µg/mL. The accuracy of the method was demonstrated by performing recovery studies from 98-102% of the nominal concentration, with a relative standard deviation (RSD) of <2%. The precision studies were assessed for system, method, intra and inter day, ruggedness was assessed yielding acceptable relative standard deviations (% RSD). Additionally, the robustness of the method was evaluated by altering parameters, confirming its reliability under different conditions. The development and validation of an analytical method was successfully applied to the analysis of Risankizumab in pharmaceutical formulations, demonstrating its suitability for identification and routine quality control analysis. To develop an eco-friendly UV spectroscopic method for estimating risankizumab, guided by white analytical chemistry principles. The method involves selecting an optimal wavelength for risankizumab detection, choosing a suitable solvent to ensure its stability with minimal interference, and validating the method for accuracy, precision, linearity, and robustness. ComplexGAPI uses a hexagonal pictogram to assess analytical methods. Analytical GREEness is based on the 12 principles of Green Analytical Chemistry (GAC), converting them into a 0–1 scale using user-friendly freeware software was developed to generate these pictograms.
Keywords
Risankizumab, UV spectroscopic method development, White analytical chemistry, ComplexGAPI, AGREE.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
