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International Journal of Zoology and Applied Biosciences Research Article
Toxicological evaluation of plant-derived therapeutics using In vitro models
Chandan Kumar D, Aswini L, Lavanya R, Sibi S and Jenifer E
Year : 2025 | Pages: 230-235
Received on: 14/09/2025
Revised on: 19/10/2025
Accepted on: 24/10/2025
Published on: 20/11/2025
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Chandan Kumar D, Aswini L, Lavanya R, Sibi S and Jenifer E( 2025).
Toxicological evaluation of plant-derived therapeutics using In vitro models
. International Journal of Zoology and Applied Biosciences, 10( 6), 230-235.
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Abstract
Plant-derived therapeutics remain a major source of bioactive compounds with significant pharmacological potential; however, their safety profiles must be rigorously evaluated before clinical use. The present study provides a comprehensive toxicological assessment of selected medicinal plant extracts using standardized in vitro models. Cytotoxicity analyses were performed using MTT and LDH assays on human epithelial and fibroblast cell lines to determine dose-response effects. Morphological evaluation, reactive oxygen species (ROS) quantification, and apoptosis/necrosis profiling were further incorporated to elucidate mechanisms of cellular toxicity. Results revealed dose-dependent variations, with some extracts demonstrating minimal cytotoxicity at therapeutic concentrations while exhibiting measurable toxicity at higher doses. The findings emphasize the importance of integrating cellular assays for establishing safety margins of plant-derived therapeutics. This toxicological evaluation underscores the need for evidence-based validation of herbal medicines and supports their safe application in drug development and traditional healthcare systems.
Keywords
Plant-derived therapeutics, Toxicological evaluation, In vitro models, Cytotoxicity, Medicinal plants.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
