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International Journal of Zoology and Applied Biosciences Review Article

Spectroscopy in biomedical diagnostics: A comprehensive systematic review

Aiswarya R, Subha C, Swathi T, Monish Raj R and B Nazreen

Year : 2025 | Pages: 77-80

doi: https://doi.org/10.55126/ijzab.2025.v10.i06.SP018

Received on: 15/09/2025

Revised on: 25/10/2025

Accepted on: 31/10/2025

Published on: 12/11/2025

  • Aiswarya R, Subha C, Swathi T, Monish Raj R and B Nazreen ( 2025).

    Spectroscopy in biomedical diagnostics: A comprehensive systematic review

    . International Journal of Zoology and Applied Biosciences, 10( 6), 77-80.

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Abstract

Spectroscopy has emerged as a powerful analytical and diagnostic tool in biomedical science, offering non-invasive, rapid, and label-free detection of biomolecular changes associated with disease. This comprehensive systematic review examines the applications of ultraviolet-visible (UV–Vis) and Fourier Transform Infrared (FTIR) spectroscopy in biomedical diagnostics. A literature survey spanning 1987 to 2024 was conducted across databases including Scopus, Web of Science, ScienceDirect, and Google Scholar. The review identifies spectroscopy’s role in disease detection, biofluid analysis, drug quality assessment, and biomolecular fingerprinting. FTIR spectroscopy demonstrates high specificity in molecular vibration analysis, while UV–Vis spectroscopy provides sensitivity in quantifying biochemical transitions. Together, these modalities support advancements in clinical chemistry, oncology, and metabolomics. Despite their diagnostic promise, challenges such as data standardization, spectral overlap, and interpretation bias remain. Future work should integrate artificial intelligence and nanotechnology to enhance diagnostic precision and clinical applicability.

Keywords

Spectroscopy, UV-Vis, FTIR, Biomedical Diagnostics, Biofluids, Molecular Fingerprinting.

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    © The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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