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International Journal of Zoology and Applied Biosciences Research Article

Phytochemical profiling and GC-MS analysis, of Anoectochilus elatus leaf extracts

Bhuvaneshwari V K, Renuka M, Balamurugan K, Kavitha R, Vijayakumar N

Year : 2026 | Volume: 11 | Issue: 1 | Pages: 259-266

doi: https://doi.org/10.55126/ijzab.2026.v11.i01.034

Received on: 27/10/2025

Revised on: 23/11/2025

Accepted on: 28/12/2025

Published on: 31/01/2026

  • Bhuvaneshwari V K, Renuka M, Balamurugan K, Kavitha R, Vijayakumar N( 2026).

    Phytochemical profiling and GC-MS analysis, of Anoectochilus elatus leaf extracts

    . International Journal of Zoology and Applied Biosciences, 11( 1), 259-266.

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Abstract

This study aimed to evaluate the phytochemical composition, identify bioactive constituents, and predict the molecular mechanisms underlying the medicinal value of Anoectochilus elatus (A. elatus) aqueous leaf extract, with particular emphasis on its potential therapeutic role against hyperammonemia-associated inflammatory and apoptotic dysfunction. Fresh leaves of A. elatus were collected, authenticated, shade-dried, powdered, and subjected to aqueous extraction. Qualitative phytochemical screening was carried out using standard chemical tests. Gas chromatography–mass spectrometry (GC–MS) was performed to determine the major phytochemical constituents. Phytochemical screening revealed the presence of flavonoids, phenolics, tannins, chlorogenic compounds, saponins, steroids, triterpenoids, and essential nutritional metabolites. GC-MS profiling identified pharmacologically relevant compounds, with 2-decanone, 2-tridecanone, benzyl benzoate, corosolic acid, and ?-sitosterol being the major constituents. The aqueous extract of A. elatus contains diverse bioactive constituents with potent antioxidant, anti-inflammatory, and apoptosis-modulating properties. Further in vitro and in vivo studies are warranted to validate their molecular effects and support the development of A. elatus-derived therapeutic interventions for hyperammonemia.

Keywords

Anoectochilus elatus, GC-MS, Linoleic acid, Medicinal orchids, Apoptosis.

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    © The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.