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International Journal of Zoology and Applied Biosciences Research Article

Received on: 12/09/2025

Revised on: 22/09/2025

Accepted on: 28/10/2025

Published on: 04/11/2025

  • Sridevi Balraj and Aiyavu Chinnaiyan( 2025).

    Neuroprotective effects of biosynthesized silver nanoparticles using diosmin in a rotenone-induced parkinson’s disease rat model

    . International Journal of Zoology and Applied Biosciences, 10( 6), 293-299.

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Abstract

This study investigated the neuroprotective potential of silver nanoparticles (AgNPs) synthesized using Diosmin against rotenone-induced behavioral and motor impairments in a male Wistar rat model of Parkinson’s disease (PD). Thirty-six male Wistar rats were randomly divided into six groups of six animals each. Group I served as the vehicle control, while Group II received intraperitoneal rotenone (0.5 mg/kg body weight). Groups III, IV, and V were co-treated with rotenone and orally administered biosynthesized AgNPs at doses of 5, 10, and 20 mg/kg, respectively, for 60 days. Group VI received AgNPs alone (20 mg/kg). Motor and behavioral performance was assessed using open field, rotarod, hanging wire, and narrow beam walking tests, among others. Rats treated with rotenone exhibited significant behavioral deficits and impaired motor coordination compared with controls (p < 0.001). Co-administration of Diosmin-based AgNPs markedly improved locomotor activity, balance, and muscle strength, restoring performance parameters close to normal levels. These findings suggest that diosmin-mediated AgNPs could serve as promising therapeutic agents for managing Parkinsonian neurodegeneration

Keywords

Rotenone, Parkinson’ s disease, Diosmin, Silver nanoparticles, Behavioral analysis, Neuroprotection.

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    © The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.