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International Journal of Zoology and Applied Biosciences Research Article
Morpho-molecular characterization of Escherichia coli and its regulation using natural spices
Shrisha R, Nandish G, Akarsh S and Thippeswamy B
Year : 2025 | Volume: 10 | Issue: 1 | Pages: 56-66
Received on: 22/12/2024
Revised on: 29/12/2024
Accepted on: 24/01/2025
Published on: 31/01/2025
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Shrisha R, Nandish G, Akarsh S and Thippeswamy B( 2025).
Morpho-molecular characterization of Escherichia coli and its regulation using natural spices
. International Journal of Zoology and Applied Biosciences, 10( 1), 56-66.
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Abstract
In present study, the effect of E. coli against different natural species were evaluated by using Kirby Bauer and the efficacy of the different natural spices against Escherichia coli was performed. The isolated E. coli strain from sewage sample which was collected from drain of Pension Mohalla located at second cross, Shivamogga town, Shivamogga district was identified based on its morphological and biochemical characters and confirmed by genomic characterization using 16s rRNA gene sequencing method and deposited to GenBank, NCBI, confirmed as Escherichia coli strain KUMBSRNGBT-113, and allocated with accession number PQ197716. Antibiotic susceptibility of different antibiotics was tested against isolated E. coli and standard E. coli by using Kirby Bauer method. Based on zone of inhibition, the isolated E. coli shows highest susceptible to cefpodoxime, (30 mm), Ciprofloxacin (28mm), Streptomycin (26mm) and Chloramphenicol (25 mm) and the isolated culture was resistant to Bacitracin, Amoxicillin, Erythromycin, Vancomycin, Ampicillin and Rifampicin. The antibacterial potency of aqueous extracts of different spices were tested against isolated and standard E. coli cultures by Agar well diffusion assay. Chloramphenicol was used as the standard antibiotic. The aqueous extract of some spices has shown antibacterial effects against the isolated Escherichia coli, Clove extract has shown highest zone of inhibition which can be described as highly effective. Dried ginger extract, Cinnamon extract, bay leaf extract has shown moderate zone of inhibition whereas Black pepper extract, coriander seeds extract and pandan leaves has shown slightly better zone of inhibition.
Keywords
Escherichia coli, Antibiotic susceptibility, Kirby Bauer method, Aqueous extract, Antibacterial effect.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
