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International Journal of Zoology and Applied Biosciences Research Article
Isolation and screening of lipase-producing microorganisms
Ramesh M, Rubala Nancy J, R Lavanya, A, A Kaaviya and B Nazreen
Year : 2025 | Pages: 17-21
Received on: 08/09/2025
Revised on: 18/10/2025
Accepted on: 25/10/2025
Published on: 10/11/2025
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Ramesh M, Rubala Nancy J, R Lavanya, A, A Kaaviya and B Nazreen( 2025).
Isolation and screening of lipase-producing microorganisms
. International Journal of Zoology and Applied Biosciences, 10( 6), 17-21.
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Abstract
Lipases (EC 3.1.1.3) are hydrolytic enzymes that catalyze the cleavage and synthesis of ester bonds in triglycerides, producing glycerol and free fatty acids. They are among the most versatile biocatalysts used across diverse industries such as food processing, detergent formulation, pharmaceuticals, leather, and biodiesel production. Microbial sources, particularly bacteria and fungi, have gained prominence due to their stability, ease of cultivation, and potential for large-scale production using low-cost substrates. The present study focuses on the isolation and screening of lipase-producing microorganisms from various environmental sources, including oil-contaminated soils, dairy effluents, and decaying organic matter. Samples were subjected to serial dilution, culture on nutrient agar supplemented with olive oil, and screening on Rhodamine B agar plates to detect lipolytic activity. Positive isolates were further characterized morphologically and biochemically to identify potential lipase producers. Preliminary results revealed that several isolates exhibited strong orange fluorescence halos under UV light, confirming extracellular lipase production. The most potent isolates were identified as belonging to the genera Bacillus, Pseudomonas, and Aspergillus. These findings demonstrate the potential of naturally occurring microorganisms as sustainable sources of lipases for industrial applications and lay the groundwork for further optimization and enzyme purification studies.
Keywords
Lipase, Microbial enzymes, Isolation, Screening, Oil-contaminated soil, Rhodamine B agar, Biocatalyst.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
