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International Journal of Zoology and Applied Biosciences Research Article
Extraction and purification of LPS from catheter-associated urinary tract infection (CAUTI) of gram-negative organisms
Sivaranjani and Karthik Sundaram
Year : 2025 | Volume: 10 | Issue: 6 | Pages: 278-284
Received on: 13/09/2025
Revised on: 16/10/2025
Accepted on: 24/10/2025
Published on: 04/11/2025
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Sivaranjani and Karthik Sundaram( 2025).
Extraction and purification of LPS from catheter-associated urinary tract infection (CAUTI) of gram-negative organisms
. International Journal of Zoology and Applied Biosciences, 10( 6), 278-284.
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Abstract
Globally, one of the most prevalent health-related illnesses is catheter-associated urinary tract infection (CAUTI). A leading cause of nosocomial infection is commonly associated with the use of catheters, leading to high morbidity and mortality rates worldwide. MDR bacteria are the main reason to leading to longer hospital stays and increased costs for treatment. Uropathogens are responsible for half of the CAUTI infections. In the pathophysiology of gram-negative bacteria, lipopolysaccharide (LPS), usually called endotoxin, is essential for colonization and invasion. It plays a crucial role in gram-negative bacteria-induced diseases and septic shock. It has broad applications in different cell stimulation experiments, providing a conceptual basis for studies directly related to the isolation and purification of LPS. This study aims to extract and purify the LPS from the Gram-negative organisms of CAUTI bacterial strains such as E. coli and P. aeruginosa. The primary approach, which is based on the hot phenol-water method extraction protocol, is used to purify LPS from bacterial strains, with high purity and minimal contamination of proteins and nucleic acids. Finally, to check the purity of the extracted LPS, it was evaluated by silver Coomassie blue staining of SDS-PAGE. The result showed that high purity of the E. coli range is 10-25 kDa; similarly, Pseudomonas aeruginosa demonstrated a banding pattern characteristic of varying O-antigen chain length. HPLC analysis reveals that the purity of the extracted LPS was 100% compared with the standard LPS.
Keywords
Urinary Tract Infection, Gram-Negative Bacteria, Multidrug Resistance, Lipopolysaccharide.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
