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International Journal of Zoology and Applied Biosciences Research Article
Extraction and identification of proteins from prostate cancer patient samples
Sowndarya GS, Rubala Nancy J, Lavanya R, Senthilkumar G P and Nazreen B
Year : 2025 | Pages: 141-144
Received on: 18/09/2025
Revised on: 20/10/2025
Accepted on: 27/10/2025
Published on: 15/11/2025
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Sowndarya GS, Rubala Nancy J, Lavanya R, Senthilkumar G P and Nazreen B( 2025).
Extraction and identification of proteins from prostate cancer patient samples
. International Journal of Zoology and Applied Biosciences, 10( 6), 141-144.
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Abstract
Prostate cancer is one of the most frequently diagnosed malignancies among men worldwide, and the identification of disease-associated proteins remains essential for biomarker discovery and improved diagnostics. The present study focuses on the extraction and identification of proteins from prostate cancer patient samples using standardized biochemical and proteomic protocols. Tissue lysates were prepared using RIPA buffer, followed by protein isolation through differential centrifugation and quantification using the Bradford assay. Extracted proteins were separated using SDS-PAGE and subsequently identified using mass spectrometry (MS/MS). The study successfully isolated high-quality protein fractions and identified several proteins previously associated with cancer progression, including PSA, α-methylacyl-CoA racemase, and heat shock proteins. These findings highlight the potential of protein profiling in distinguishing cancerous from non-cancerous tissue and support the role of proteomics in prostate cancer biomarker discovery.
Keywords
Prostate cancer, Proteomics, Protein extraction, Biomarkers, SDS-PAGE, Mass spectrometry.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
