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International Journal of Zoology and Applied Biosciences Research Article
Exploring the antimicrobial attribute: Characterization of lactobacillus isolate and in vitro assessment of antimicrobial activity of postbiotic cell-free supernatants against pathogenic microorganisms
Arunavarsini Kumarasamy and R Mahenthiran
Year : 2025 | Volume: 10 | Issue: 5 | Pages: 83-88
Received on: 31/07/2025
Revised on: 12/08/2025
Accepted on: 13/09/2025
Published on: 30/09/2025
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Arunavarsini Kumarasamy and R Mahenthiran( 2025).
Exploring the antimicrobial attribute: Characterization of lactobacillus isolate and in vitro assessment of antimicrobial activity of postbiotic cell-free supernatants against pathogenic microorganisms
. International Journal of Zoology and Applied Biosciences, 10( 5), 83-88.
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Abstract
This study examines the antimicrobial potential of the cell-free supernatant of a Lactobacillus isolate. The isolate was identified accurately by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial potential against four common pathogenic bacteria, namely Escherichia coli, Enterococcus faecalis, Salmonella enterica, and Streptococcus bovis, was determined by agar well diffusion assay. The results demonstrated concentration-dependent increases in inhibition zones for all tested pathogens; the postbiotic showed significant antimicrobial potential similar to reference antibiotics. This study demonstrates the potential of probiotic cell-free supernatant as a bioactive postbiotic agent that can inhibit pathogenic bacteria, thus providing alternative infection control. The results also suggest the potential of the postbiotic in being developed as a bioactive antimicrobial agent for infection management.
Keywords
Yogurt, Postbiotic, Cell free supernatant, Pathogenic bacteria and Antimicrobial activity.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
