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International Journal of Zoology and Applied Biosciences Research Article
Evaluation of anticancer activity Tinospora cordifolia leaves extract and its isolated fractions
Vrushali Tambe, Rohini Suralkar, Archana Karnik, Anjali Kshirsagar, Vijaya Vichare and Shama Aphale
Year : 2026 | Volume: 11 | Issue: 1 | Pages: 1-7
Received on: 15/10/2025
Revised on: 28/11/2025
Accepted on: 20/12/2025
Published on: 01/01/2026
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Vrushali Tambe, Rohini Suralkar, Archana Karnik, Anjali Kshirsagar, Vijaya Vichare and Shama Aphale( 2026).
Evaluation of anticancer activity Tinospora cordifolia leaves extract and its isolated fractions
. International Journal of Zoology and Applied Biosciences, 11( 1), 1-7.
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Abstract
The study investigated the anticancer potential of phytoconstituents isolated from Tinospora cordifolia, an established medicinal plant used in traditional medicine. The authenticated plant leaves were extracted with ethanol, and the crude extract along with its toluene fraction was analyzed for phytochemical constituents. Qualitative tests confirmed the presence of alkaloids, steroids, glycosides, and flavonoids. Separation through chromatographic techniques resulted in three distinct fractions. The anticancer potential of the extract and fractions was evaluated on human cervical and lung cancer cell lines using standard cell viability assays. The crude ethanolic extract exhibited considerable cytotoxic activity, while among the isolated fractions, the third fraction showed pronounced inhibitory effects on cancer cell growth comparable to the standard reference compound. LC–MS/MS profiling of the active fraction indicated the presence of magnoflorin, bergenin, stigmasterol, and 8-hydroxy tinosporide. The findings suggested that Tinospora cordifolia contains bioactive phytoconstituents with significant anticancer potential. The study concluded that these constituents may act synergistically to inhibit cancer cell proliferation and could serve as leads for the development of safer, plant-based anticancer agents.
Keywords
Tinospora, Alkaloids, Antineoplastic potential, Bioactive phytoconstituents, Cytotoxic evaluation.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
