Archives
International Journal of Zoology and Applied Biosciences Research Article
Enhanced production of lipase by marine Bacillus subtilis through statistical optimization of fermentation parameters
Nirmala B, Swathi T, Sowmiya B, Anandhi A and Sujitha K
Year : 2025 | Pages: 7-11
Received on: 07/09/2025
Revised on: 17/10/2025
Accepted on: 25/10/2025
Published on: 10/11/2025
-
Nirmala B, Swathi T, Sowmiya B, Anandhi A and Sujitha K( 2025).
Enhanced production of lipase by marine Bacillus subtilis through statistical optimization of fermentation parameters
. International Journal of Zoology and Applied Biosciences, 10( 6), 7-11.
-
click to view the cite format
Abstract
Lipases are industrially significant enzymes that catalyze the hydrolysis and synthesis of esters, playing a vital role in diverse biotechnological sectors including food processing, detergent formulation, and biodiesel production. The present study focuses on the enhanced production of lipase by a marine-derived strain of Bacillus subtilis through optimization of fermentation parameters. The isolate was obtained from seawater samples collected along the coastal region and identified through morphological and biochemical characterization. Various process parameters such as carbon and nitrogen sources, pH, temperature, inoculum size, and incubation time were optimized to maximize enzyme yield. Statistical optimization using Response Surface Methodology (RSM) was employed to determine interactive effects among the key variables. The optimized conditions resulted in a 2.5-fold increase in lipase activity compared to unoptimized conditions, achieving a maximum specific activity of 16.8 U/mL. The enzyme exhibited stability across a wide pH (6.0–9.0) and temperature range (30–60°C), confirming its potential for industrial applications. The results highlight the potential of marine Bacillus subtilis as a robust source of lipase with desirable catalytic properties for bioprocess and biorefinery industries.
Keywords
Lipase, Bacillus subtilis, Marine bacteria, Statistical optimization, Response surface methodology.
-
Full Article PDF (
13)
- View HTML Article
Copy Rights
© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
