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International Journal of Zoology and Applied Biosciences Research Article
Detection and classification of chemokine receptor positive immune cells
Maniraj K, Saalini Vellivel , Swathi T , Senthilkumar G P and Sujitha K
Year : 2025 | Pages: 419-422
Received on: 25/09/2025
Revised on: 20/10/2025
Accepted on: 23/11/2025
Published on: 03/12/2025
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Maniraj K, Saalini Vellivel , Swathi T , Senthilkumar G P and Sujitha K( 2025).
Detection and classification of chemokine receptor positive immune cells
. International Journal of Zoology and Applied Biosciences, 10( 6), 419-422.
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Abstract
Chemokine receptors orchestrate immune cell trafficking, positioning, and activation, making their detection crucial for understanding immune responses and inflammatory disorders. This study aimed to identify and classify chemokine receptor–positive immune cells using multicolor flow cytometry with simulated human peripheral blood mononuclear cells (PBMCs). A 12-color staining panel targeting major chemokine receptors (CCR2, CCR5, CCR7, CXCR3, and CXCR4) was developed and validated. Data acquisition and analysis were performed using standardized gating strategies, including lymphocyte identification, doublet exclusion, viability gating, and lineage subset discrimination (T cells, B cells, NK cells, and monocytes). The results indicated heterogeneous but receptor-specific expression patterns across immune subsets. Memory T cells exhibited high CCR7 and CXCR3 expression, while inflammatory monocytes were enriched for CCR2 and CCR5. The study demonstrates that multicolor flow cytometry provides robust, high-resolution classification of chemokine receptor–positive immune cell populations, supporting its utility in immunophenotyping, disease biomarker discovery, and therapeutic targeting.
Keywords
Chemokine receptors, Flow cytometry, Immune cells, Immunophenotyping, CCR.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
