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International Journal of Zoology and Applied Biosciences Research Article
Characterization and estimation of phytochemical constituents in traditional Indian medicinal flora
Sathish R, Boopathy G, Devasena B, Sowmiya B and Jenifer E
Year : 2025 | Pages: 514-517
Received on: 27/09/2025
Revised on: 24/10/2025
Accepted on: 25/11/2025
Published on: 01/12/2025
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Sathish R, Boopathy G, Devasena B, Sowmiya B and Jenifer E( 2025).
Characterization and estimation of phytochemical constituents in traditional Indian medicinal flora
. International Journal of Zoology and Applied Biosciences, 10( 6), 514-517.
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Abstract
Indian medicinal plants are rich sources of bioactive phytochemicals with therapeutic relevance in modern drug development. This study aims to characterize and quantify major phytochemical constituents from selected medicinal plants traditionally used in Ayurveda and Siddha systems. Standard extraction procedures using methanol, ethanol, and aqueous solvents were optimized. Qualitative phytochemical screening was conducted for alkaloids, flavonoids, tannins, terpenoids, saponins, phenols, and glycosides. Quantitative estimation of key metabolites, including total phenolic content (TPC), total flavonoid content (TFC), and alkaloid concentration, was performed using Folin-Ciocalteu, aluminum chloride, and Harborne methods, respectively. Further characterization using FTIR, UV Vis spectrophotometry, and HPLC revealed significant variation in metabolite profiles among plant species. The results confirm the presence of diverse secondary metabolites with strong medicinal potential. These findings provide a scientific basis for the therapeutic value of Indian medicinal plants and their possible applications in herbal formulations and pharmaceutical industries.
Keywords
Phytochemical characterization, Indian medicinal plants, Secondary metabolites, HPLC.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
