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International Journal of Zoology and Applied Biosciences Research Article
Biomedical applications of green synthesized silver nanoparticles from Prosopis juliflora
Manjunatha D, Kruthika P, Chethana MM, Sinchana GC, Mohammed Sohail, Kavya SP and Prakash J
Year : 2025 | Volume: 10 | Issue: 4 | Pages: 107-121
Received on: 27/05/2025
Revised on: 11/06/2025
Accepted on: 29/06/2025
Published on: 31/07/2025
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Manjunatha D, Kruthika P, Chethana MM, Sinchana GC, Mohammed Sohail, Kavya SP and Prakash J( 2025).
Biomedical applications of green synthesized silver nanoparticles from Prosopis juliflora
. International Journal of Zoology and Applied Biosciences, 10( 4), 107-121.
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Abstract
The present study explores the phytochemical properties and green synthesis of silver nanoparticles using the ethanol extract of Prosopis juliflora, a medicinal plant known for its bioactive constituents. Plant samples were aseptically collected from the Davangere district, Karnataka, and processed through washing, shade drying, and grinding. The crude extract was obtained using cold maceration with ethanol, followed by filtration and drying. Qualitative phytochemical screening confirmed the presence of secondary metabolites including phenols, tannins, saponins, and glycosides. UV-Visible spectrophotometry revealed an absorption peak at 434 nm, indicating the presence of bioactive compounds. FTIR spectroscopy identified functional groups involved in the biochemical profile of the extract. GC-MS analysis detected 35 secondary metabolites in the extract, with major constituents such as Ethane, 1,1-diethoxy-, Propane, 2,2-diethoxy-, and 4-O-Methylmannose. The green synthesis of silver nanoparticles was achieved by adding 1 mM AgNO? to the ethanol extract, confirmed by a color change from light brown to dark brown. The synthesized Pj-AgNPs were characterized by UV-Vis spectroscopy, showing a sharp peak at 401 nm. FTIR analysis of the nanoparticles revealed key functional groups involved in stabilization. SEM images confirmed spherical morphology, and EDAX analysis detected elemental silver along with carbon and oxygen. DLS analysis showed an average nanoparticle size of 70.21 nm. Antibacterial assays using the agar well diffusion method demonstrated significant inhibition, with the extract showing the highest activity against Enterococcus faecalis (25 mm) and Pj-AgNPs most effective against Salmonella typhi (19 mm). The findings suggest potential biomedical applications of Pj-AgNPs as natural antimicrobial agents.
Keywords
Prosopis juliflora, GC-MS, Pj-AgNPs, Antibacterial activity.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
