Archives
International Journal of Zoology and Applied Biosciences Research Article
Apoptotic induction and ros-mediated cytotoxic mechanism of Allium ascalonicum extract against HEPG2 liver cancer cells
Kumaresan Anbu, Vijai Krishna V, Anitha, W, Menaka T and Sujitha K
Year : 2025 | Pages: 194-200
Received on: 11/09/2025
Revised on: 19/10/2025
Accepted on: 25/10/2025
Published on: 20/11/2025
-
Kumaresan Anbu, Vijai Krishna V, Anitha, W, Menaka T and Sujitha K( 2025).
Apoptotic induction and ros-mediated cytotoxic mechanism of Allium ascalonicum extract against HEPG2 liver cancer cells
. International Journal of Zoology and Applied Biosciences, 10( 6), 194-200.
-
click to view the cite format
Abstract
Allium ascalonicum (Shallot) possesses a wide spectrum of bioactive compounds with promising therapeutic properties, including anticancer potential. This study investigates the cytotoxic and apoptotic effects of A. ascalonicum ethanolic extract on HepG2 human liver cancer cells, emphasizing the role of reactive oxygen species (ROS) in mediating cell death. The extract exhibited selective cytotoxicity toward HepG2 cells while showing minimal toxicity to normal hepatic cells, as confirmed by MTT assay. Morphological assessment and fluorescence microscopy revealed hallmark features of apoptosis, such as nuclear condensation and membrane blebbing. Biochemical analyses demonstrated increased intracellular ROS generation and disruption of mitochondrial membrane potential, leading to the activation of caspase-dependent apoptotic pathways. These findings suggest that A. ascalonicum ethanolic extract exerts its anticancer effects primarily through oxidative stress-induced apoptosis. The results highlight the therapeutic potential of A. ascalonicum as a natural source of anticancer agents and support further in vivo and molecular investigations to explore its applicability in hepatocellular carcinoma treatment.
Keywords
Allium ascalonicum, HepG-2 cells, Vero cells, MTT assay, DAPI staining, DCFDA staining, ROS, Apoptosis.
-
Full Article PDF (
14)
- View HTML Article
Copy Rights
© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
