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International Journal of Zoology and Applied Biosciences
A pilot study on the isolation and identification of histamine-producing bacteria from narrow barred spanish mackerel and japanese thread fin bream from Ukkadam fish market, Coimbatore, India
Vinisha Kartheesan, Mohammed Rafi L, Anju Alagiri, Velayutham Meiyalagan
Year : 2023 | Volume: 8 | Issue: 6 | Pages: 96-105
Received on: 13/11/2023
Revised on: 27/11/2023
Accepted on: 17/12/2023
Published on: 31/12/2023
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Vinisha Kartheesan, Mohammed Rafi L, Anju Alagiri, Velayutham Meiyalagan( 2023).
A pilot study on the isolation and identification of histamine-producing bacteria from narrow barred spanish mackerel and japanese thread fin bream from Ukkadam fish market, Coimbatore, India
. International Journal of Zoology and Applied Biosciences, 8( 6), 96-105.
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Abstract
Consumption of marine fishes with high histamine levels due to improper storing leads to cause scombroid or histamine fish poisoning. In spoiled fishes, bacteria begin to break down the histidine into histamine by decarboxylation activity. Previous studies reported that black muscle fishes usually produce large amounts of histamine-producing bacteria (HPB). In this study, two white muscle fishes such as Scomberomorus commerson (Narrow-barred Spanish mackerel) and Nemipterus japonicus (Japanese threadfin bream) were chosen to detect the presence of histamine-producing bacteria (HPB). The fish samples were purchased from Ukkadam fish market in Coimbatore and examined for the occurrence of HPB on Niven agar. The isolated HPB on media were analyzed for identification based on their morphological characteristics, primary test, and biochemical test. Finally, 8 colonies were confirmed as the cause for histamine production of both fishes and among them, 5 belong to the family Enterobacteriaceae and others belong to the family Aeromonadaceae, Morganellaceae, and Enterococcaceae. Our study showed that the histamine-producing bacteria highly reported in dark-muscle fishes were also isolated from the chosen white-muscle fishes. Hence the presence of HPB indicates the improper maintenance of fish in retail fish markets. This considerable incidence of histamine-producing bacteria which on proliferation under suitable conditions may contribute to toxic histamine accumulation in the flesh of fishes.
Keywords
Histamine-producing bacteria, Niven agar, Nemipterus japonicus, Scomberomorus commerson.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
