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International Journal of Zoology and Applied Biosciences Research Article
Biohydrogen production through dark fermentation by soil-derived Bacillus pumilus
Paul Beaulah BF, Lakshmi Prabha, Monika Y, Amritha Sathya P, Srinivasan P, Rajalakshmi M
Year : 2026 | Volume: 11 | Issue: 2 | Pages: 1-8
Received on: 27/12/2025
Revised on: 09/01/2026
Accepted on: 07/02/2026
Published on: 01/03/2026
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Paul Beaulah BF, Lakshmi Prabha, Monika Y, Amritha Sathya P, Srinivasan P, Rajalakshmi M( 2026).
Biohydrogen production through dark fermentation by soil-derived Bacillus pumilus
. International Journal of Zoology and Applied Biosciences, 11( 2), 1-8.
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Abstract
The search for renewable and eco-friendly energy alternatives has led to increased interest in microbial hydrogen production. In this study, soil samples from agricultural fields were analysed to isolate hydrogen-producing bacteria. The potential isolate, identified as Bacillus pumilus, exhibited strong amylase activity, enabling effective degradation of complex carbohydrates into fermentable sugars for dark fermentation. Hydrogen generation was carried out using organic waste substrates under controlled anaerobic conditions. GC–MS analysis confirmed hydrogen as the predominant gaseous product with minimal methane contamination. These findings demonstrate the capability of Bacillus pumilus as a robust, low-cost, and sustainable candidate for biological hydrogen production using agricultural residues.
Keywords
Biohydrogen, Dark fermentation, Bacillus pumilus, Amylase, Anaerobic bioreactor, Renewable energy.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
