Abstract

Enzyme assay was made to amylase, protease, lipase, cellulase, acid phosphatase and alkaline phosphatase in different zones of the  gastrointestinal tract and digestive gland of the land snail Cryptozona bistrialis in summer and monsoon season. The amylase activity ranged from 2.46 to 8.42 IU/mg/protein. Protease varied from 1.69 to 3.90 µ mole tyrosine /mg/ protein/ hr. Lipase activity ranged from 1.01 to 5.01 µ mole tyrosine/ mg/ protein/ hr. The total activity of cellulase ranged between 0.74 and 13.12 μ mole tyrosine/mg/protein/hr. The level of acid phosphatase ranged from 1.7 to 4.03 µ mole p-nitrophenol/ mg/ protein /hr and alkaline phosphatase level ranged from 3.27 to 4.90 µ mole/ p-nitrophenol/ mg/ protein/ hr. The highest activity of enzymes were noticed in the digestive gland and lowest activity was found in stomach in both seasons. It was also observed that the land sail C.bistrialis showed strong amylase activity followed by alkaline phosphatase. Seasonally the maximum activity of enzymes were found in monsoon and minimum was in summer.

Keywords

Enzyme activity, Gastrointestional tract, Digestive gland, Cryptozona bistrialis, Seasonal changes.

Received on 13/07/2016, Accepted on 29/08/2016, Published on 03/04/2017