Abstract

The solid state fermentation (SSF) has been reputable as a superior technique for the enzyme production. The present study targeted on the amylase production from Bacillus amyloliquefaciens and Aspergillus niger by using different agro industrial waste like sago waste, rice bran and wheat bran as substrate with the use of SSF techniques and tested for its activity. The effect of different parameters such as time, pH and temperature were also tested. B. amyloliquefaciens and A. niger was identified as the best producers of amylase at pH 7 and maximum production was in rice bran and wheat bran. Substrates tested for amylase production could be effectively exploited in the future production of various commercial products.

Keywords

Solid state fermentation, Amylase, Wheat bran, Sago waste, Rice bran.

Received on 11/10/2016, Accepted on 15/11/2016, Published on 31/12/2016