Abstract

Vorticella stalk is a highly contractile device exclusively used to know the behavior of motor proteins (spasmins & batonnets) for new information in the light of thermodynamics. For the same purpose preapproved materials and methods were used to reflect enzyme – kinetics by work, power and force measurement through applying equation of motion, mean and standard deviation, where in case of Vorticella stalk contraction, Vmax became constant at initial stages of mechanical motion but presented upper-limit burst prediction in the presence of DNFB (2,4 – dinitro – flouro – benzene) from 1 mM to 5 mM. Birefringence variation was 5 to 8 % for pCa. Binding affinity variables were 1 to 2% for batonnets in comparison to spasmins, whereas it was 5 to 6% for myosin in the presence of DNFB. Denominators and numerators were well designed coordinates for geometric explanation with equation of motion. Kcat was prevented by reversible reactions at pHs 5 to 6.8.

Keywords

Vorticella stalk, Mechanical motion, Birefringence, Kcat.

Received on 02/01/2020, Accepted on 26/01/2020, Published on 05/02/2020