Abstract

Reverse transcription polymerase chain reaction is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction  It is primarily used to measure the amount of a specific RNA. Attempt deals with detection of grasserie virus, BmNPV in the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) through polymerase chain reaction. The mulberry silkworm, Bombyx mori L is a Lepidopteran insect, life cycle of which includes: Egg, Larval Instars, Pupa within silky cocoon and adult moth. It is purely domesticated insect since long, which make it a quite delicate venture, easily susceptible to viral and other diseases. The viral diseases are difficult to manage due to a very short life cycle of silkworm. One of the most effective solutions is a timely detection of such infection so that to stop spread of the disease.  The present attempt is concerned with studies on a polymerase chain reaction (PCR) with a set of specific primers to the Grasserie virus gene region was used to diagnose B. mori  nucle-opolyhedro-virus (BmNPV). The nucleic acid DNA was extracted from the mid gut tissue of the fifth instar larvae of silkworms and was subjected for amplification. After the amplification the samples were loaded on 1% Agarose gel and electrophoresis was run at 65 volts. The gel was stained using stain (ethidium bromide) and used to visualize under UV illuminator. The results of the amplification of the polymerase chain reaction were utilized for the detection of infection of Grasserie BmBPV

Keywords

Silkworm, Bombyx mori L, Viral Diseases, Nucleopolyhedrosis virus (NPV).

Received on 27/02/2019, Accepted on 12/03/2019, Published on 22/03/2019