Abstract

Corpora lutea of normal (11-15 days) and regressing category (16-21 days) of goat were analysed for morphological and biochemical variations. For biochemical studies, total lipids cholesterol content and proteins were estimated. Corpus luteum was comprised of two types of steroidogenic cells viz Granulosa luteal cells and Theca luteal cells. Ultrastructurally, regressing corpus luteum revealed disorganization of luteal cells with shrunken cytoplasm, uneven nuclear membrane, increased number of lipid droplets and lysosomes, apoptotic granules, disintegrating and vacuolated rough endoplasmic reticulum and swollen mitochondria as compared to normal corpus luteum.  The total lipid content was less in normal corpus luteum (1.840 ± 0.52 mg/100 mg) and more in regressing corpus luteum (3. 108 ± 0.84 mg/100 mg). The value of cholesterol was more in regressing (2.683±0.31mg/100 mg) as compared to large category (.315 ± 0.04mg/100 mg). Whereas the value of protein content of normal corpus luteum was found to be more (19.55 ± 3.45 mg/g wt. of the tissue) as compared to regressing corpus luteum (17.46 ± 1.70 mg/g wt. of the tissue). Ultrastructurally presence of lysosomes during regression corresponds to the autophagocytotic bodies with minimum progesterone level. During regression maximum amount of lipids and cholesterol shows less progesterone production as compared to normal corpus luteum whereas lesser content of protein during regression is related to lesser production of steroidogenic enzymes.

Keywords

Corpus Luteum, Goat Ovary, Lipids, Cholesterol, Proteins.

Received on 31/01/2018, Accepted on 27/02/2018, Published on 04/03/2018