Abstract

Mosquitoes are serious threat to public health through which several dangerous diseases are transmitted in both animals and human beings. The mosquito Culex quinquefasciatus is a significant vector of the filarial nematode, Wuchereria bancrofti, and cause Lymphatic filariasis. The residual spray of insecticides in the control of vector borne diseases is limited. Majority of the chemical pesticides are harmful to man and animals, some of which not easily degradable and spreading toxic effects. In recent years interest in plant based products has been revived because of the development resistance, cross-resistance and possible toxicity hazardous associated with synthetic insecticides and the rise of their cost. In the present study molecular docking analysis were performed using secondary metabolites selected from the plant Kalanchoe pinnata against the glutathione-s-transferase protein of Wuchereria bancrofti. Result suggested that among the four bioactive compounds viz., vitamin D, beta-amyrin, alpha-amyrin and stigmasterol bonded with the glutathione –S-transferase protein (PDB ID:5D73) producing a good glide score. Whereas the four compounds, the partition coefficient (QPlogPo/w) and water solubility (QPlogS), critical for estimation of absorption and distribution of drugs within the body ranged between 6.941 to 8.968 and -7.261 to -8.476. Cell permeability (QPPCaco2), a key factor governing drug metabolism and its access to biological membranes, ranged from 3413 to 4557, QPPMDCK ranges from 2548M to 2478. Overall, the percentage human oral absorption for the compounds ranged 100%. All these pharmacokinetic parameters are within the acceptable range defined for human use, thereby indicating their potential as drug-like molecules.

Keywords

Culex quinquefasciatus, Kalanchoe pinnata, Lymphatic filariasis, Molecular docking. Pi-Class Glutathione- S- transferase, Wuchereria bancrofti.

Received on 03/08/2017, Accepted on 17/10/2017, Published on 28/10/2017