Abstract

Pesticides are stable compounds and they enter the aquatic ecosystem through the agriculture run off. The evaluation of nature and degree of harmful effects produced by the toxic substance in the aquatic organisms are evaluated by toxic tests. 96 hour LC50 values have generally been found to be satisfactory for the measurement of acute toxicity. Therefore, in this present study is an attempt to study the toxicity of the pesticide with respect to the hematology, of fish Oreochrommis mossambicus.The Chlorpyrifos affects not only fish but also organisms in the food chain through the process of consumption of one by the other. The pesticide enters the body tissues of the fish that affects physiological activities. The cytometric measurements of erythrocytes of sublethal exposure showed that there are not many differences from the control. In the control fish, the erythrocytes were oval in shape with elongated nucleus. Fish exposed to sublethal concentration of Chlorpyrifos showed abnormal size Reduction in the volume of the cytoplasm of cells and swelling of nuclei were observed in fish exposed to concentration. In the hematology, the total Red Blood Corpuscle and Haemoglobin content were decreased with the increasing hours of exposure of the chlorpyrifos 36% EC. The amount of the Mean Corpuscular Haemoglobin (MCH) also was increased. The constant increase in the differential count clearly indicates that the pesticide stress certainly stimulates the white blood cells to produce more at all times of exposure. It has been suggested that the enumeration of differential cell ratio counts provision of useful diagnostic procedure to assess the physiological stress in the fish.

Keywords

Tribulus terrtri, chlorpyrifos, Haematology, Oreochrommis mossambicus.

Received on 17/04/2017, Accepted on 15/10/2017, Published on 27/10/2017