Abstract

Laboratory activities of crude (hexane, ethyl acetate, chloroform and methanol) extract of Pedalium murex have been assessed in the present study. The fourth instars larvae were showing to concentrations (50-250 ppm) of methanol, chloroform, ethyl acetate and hexane leaf extracts of P. murex plant. The mortality was evidenced after 24 hrs exposure and LC50 and LC90 were determined. The ovicidal activity was resolute against different mosquito species to different concentration variety from 60–360 ppm lower than the laboratory condition. The repellent activity of P. murex extracts tested at concentration 3.5 mg/cm2 was appraised in a net cage (45cm × 30cm × 45cm) containing 100 blood ravenous female mosquitoes of Ae. aegypti and Cx. quinquefasciatus. The current investigation discovered that the LC50 values methanol extract of P. murex against Cx. quinquefasciatus followed by Ae. aegypti larvae were 111.66 and 127.08 mg/L respectively. Highest concentrations 300 and 360 ppm of extract exhibited 100% ovicidal activity. It demonstrated that repellency depends on the potency of the 3.5 mg/cm2 provided 100% protection up to 200 and 240 min against Cx. quinquefasciatus and Ae. aegypti respectively. The repellent action of methanol extract P. murex to be the majority effectual and the maximum activity was observed at 3.5 mg/cm2 concentration given 100% protection up to 200 and 240 min against Cx. quinquefasciatus and Ae. aegypti. The results evidently demonstrate that larvicidal, ovicidal and repellent activity was dosage reliant. The highest larvicidal, ovicidal and repellent activity against Cx. quinquefasciatus was gated with methanol extract of P. murex. 

Keywords

Pedalium murex , Aedes aegypti, Culex quinquefasciatus, Larvicidal, Ovicidal, Repellent activity.

Received on 11/02/2016, Accepted on 27/02/2016, Published on 29/02/2016