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International Journal of Zoology and Applied Biosciences

Year : 2018 | Volume: 3 | Issue: 5 | Pages: 375-381

doi: https://doi.org/10.5281/zenodo.1439290

Effect of different solvents system on antioxidant activity and phytochemical screening in various habitats of Ocimum basilicum L. (Sweet Basil) leaves

M. Murali and G. Prabakaran

Received on: 11/27/2017

Revised on: 04/20/2018

Accepted on: 04/23/2018

Published on: 09/29/2018

  • M. Murali and G. Prabakaran( 2018).

    Effect of different solvents system on antioxidant activity and phytochemical screening in various habitats of Ocimum basilicum L. (Sweet Basil) leaves

    . International Journal of Zoology and Applied Biosciences, 3( 5), 375-381.

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Abstract

The present study is to screen the antioxidant activity and phytochemical constituents of different polar and non polar solvent such as methanol, acetone, Petroleum ether, chloroform and aqueous extracts of various geographic habitat Ocimum basilicum leaves. Soxhlet extraction of leaves was used to find the antioxidant activity performed using non enzymatic ABTS and enzymatic GPx assay. Based on the results of antioxidant content present in Basil leaves methanol extracts further study was made to find the qualitative phytochemical analysis, Thin Layer Chromatography and GC-MS. In the antioxidant activity determined by ABTS radical scavenging method the highest value was highest observed in the lowest concentration (0.5 mg/ml) of methanol and aqueous extracts 42.03 ± 0.05 and 41.96 ± 96 respectively in Chidambaram and Yercaud samples. Glutathione peroxidase least concentration (0.5 mg/ml) of aqueous extract from Ramanathapuram sample found to show the (176.26 ± 0.05 units/mg protein/min) highest activity. Phytochemical screening was performed to find the presence of alkaloids, flavonoids, terpenoids moderate concentrations, polyphenols, saponins, steroids and negative result glycosides and tannins. In GC-MS analysis qualitatively more than ten bioactive compounds were identified including Linalool, Caffeic acid etc. Results of the present study on O. basilicum has promising antioxidant activity and could serve as natural antioxidants.

Keywords

Antioxidant activity, ABTS, GPx, Ocimum basilicum, Phytochemicals, GC-MS.